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Calcium Signalling
A Practical Approach
New Edition
Edited by Alexei Tepikin
250 pages
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12 colour plates, numerous figures
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246x189mm
978-0-19-963848-2
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Hardback
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21 December 2000
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This item is printed to order. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Many of the authors actually developed the methods described
- Well illustrated containing 12 colour plates
This new edition of Calcium Signalling focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calcium extrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc
and transition metals and a chapter is devoted to their detection and minimization. Some of the pharmacological methods of studying calcium release are also covered. Many of the chapters are written by the scientists who developed the methods they are describing so the authors could not be more qualified. It is hoped that this volume will prove as invaluable to the studying cellular calcium as its predecessor.Readership: Any researcher in the biomedical sciences interested in the role of calcium in cellular regulation.
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Edited by Alexei Tepikin, Physiological Laboratory, University of Liverpool Contributors:
Atsushi Miyawaki, Japan; Juan Llopis, Howard Hughes Medical Institute, University of California, USA; Hideaki Mizuno, Walko-city, Japan; Kees Jelink, Department of Cell Biology, The Netherlands Cancer Institute; Roger Y. Tsien, Howard Hughes Medical Institute, University of California, USA; Peter Lipp, Martin D. Bootman, Tony Collins, Laboratory of Molecular Signalling, The Babraham Institute, Cambridge; Vladislav Snitsarev, Department of Internal Medicine, University of Iowa, USA; Alan R. Kay, Department of Biological Sciences, University of Iowa, USA;
Paulo J. Magalhaes, Paolo Pinton, Luisa Filippin, Anna Chiesa, Tullio Pozzan, University of Padua, Department of Biomedical Sciences, Italy; Marisa Brini, University of Padua, Department of Biochemistry, Italy; Rosario Rizzuto, Department of Biomedical Sciences, University of Padua, Italy; Mart H. Mojet, D. Jake Jacobson, Julie Keelan, Olga Vergun, Michael R. Duchen, Physiology Imaging Consortium, Department of Physiology, London; Aldebaran M. Hofer, Harvard Medical School Brigham and Women's Hospital and the West Roxbury VAMC, USA; Oleg Gerasimenko, Julie Gerasimenko, The Physiological Laboratory, The University of Liverpool; Ricardo E. Dolmetsch, Division of Neuroscience, Children's Hospital, Harvard Medical School, USA; Paul A. Negulescu, Aurora Biosciences, USA; Lawrence D. Gaspers,
Andrew P. Thomas, Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, USA; Sandip Patel, Department of Pharmacology, University of Oxford; Anthony J. Morgan, Centre for Cardiovascular Biology and Medicine, King's College London; Paul A. Anderson, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, USA; Antony Galione, Justyn Thomas, Grant Churchill, Department of Pharmacology, University of Oxford; Alexei Tepikin, The Physiological Laboratory, The University of Liverpool
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""The book provides frank discussions about the advantages and disadvantages of a wide range of approaches in calcium signalling. It will be essential for new researchers in the field and provides useful new information for established workers. Also, the clearly presented protocols should be easy to follow in the lab." Trends in Cell Biology"
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Chameleons as cytosolic and intra-organellar calcium probes
Photometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studies
Detecting and minimizing errors in calcium-probe measurements arising from transition metals and zinc
Targeting of bioluminescent probes and calcium measurements in different subcellular compartments
Monitoring mitochondrial function in single cells
Using low-affinity fluorescence calcium indicators and chelators for monitoring and manipulating free (Ca2+) in endoplasmic reticulum.
Measuring calcium in the nuclear envelope and nucleoplasm
From calcium signal to gene expression. Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells.
Monitoring the generation and propagation of calcium signals in multicellular preparations.
Pharmacological studies of new calcium release mechanisms
Measuring calcium extrusion
Appendix one - Fluorescent calcium indicators and caged calcium probes
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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