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Readership: Any researcher in the biomedical sciences interested in the role of calcium in cellular regulation.
Edited by Alexei Tepikin, Physiological Laboratory, University of Liverpool
""The book provides frank discussions about the advantages and disadvantages of a wide range of approaches in calcium signalling. It will be essential for new researchers in the field and provides useful new information for established workers. Also, the clearly presented protocols should be easy to follow in the lab." Trends in Cell Biology"
Chameleons as cytosolic and intra-organellar calcium probes
Photometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studies
Detecting and minimizing errors in calcium-probe measurements arising from transition metals and zinc
Targeting of bioluminescent probes and calcium measurements in different subcellular compartments
Monitoring mitochondrial function in single cells
Using low-affinity fluorescence calcium indicators and chelators for monitoring and manipulating free (Ca2+) in endoplasmic reticulum.
Measuring calcium in the nuclear envelope and nucleoplasm
From calcium signal to gene expression. Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells.
Monitoring the generation and propagation of calcium signals in multicellular preparations.
Pharmacological studies of new calcium release mechanisms
Measuring calcium extrusion
Appendix one - Fluorescent calcium indicators and caged calcium probes