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Phage Display
A Practical Approach
Edited by Tim Clackson and Henry B. Lowman
360 pages
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48 Line figures, 1 halftone and 14 Tables
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246x189mm
978-0-19-963874-1
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Hardback
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04 March 2004
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This item is printed to order. Items which are printed to order are normally despatched and charged within 5-10 days.
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- A single point of reference for all aspects of this often daunting, multi-step technology
- "Modular" chapters on making libraries, performing selections, and analyzing clones
- Extensively cross-referenced
- Draws on work from a variety of leading labs in the field
Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display- A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a
library to performing selections and analyzing the results. An introductory chapter provides an overview of phage biology and phage display, including guidelines for planning a successful phage display experiment. Individual chapters provide protocols for constructing libraries using oligonucleotide-directed mutagenesis or DNA recombination, performing binding selections, and analyzing the binding activities of selected phage clones. Separate chapters then cover common applications, including selection of ligands from peptide libraries, generation of phage antibody libraries and isolation and optimization of antibodies, selection of DNA binding proteins, and expression cloning using cDNA display. Further chapters describe alternative selection strategies, such as selection using immune
sera, selection based on enzymatic activity or protein stability, and selection in vivo. Protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples given to customize the procedures to their own protein or selection system of interest. Written by experts in the field, Phage Display- A Practical Approach provides a comprehensive guide to the design and execution of phage display projects, for all those using the technique in basic research and drug discovery. Readership: 1) Graduate students and postdocs embarking on a phage display project
2) Principal Investigators who are considering phage display projects or who are relatively new to the field-could be basic researchers, clinical researchers, or researchers in drug discovery (biotech/pharma)
3) Established phage display users 4) Curious chemists
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Edited by Tim Clackson, ARIAD Pharmaceuticals, Inc., and Henry B. Lowman, Genentech, Inc. Contributors:
Frances H. Arnold, Marcus D. Ballinger, Andrew Bradbury, Yen Choo, Tim Clackson, Riccardo Cortese, Brian C. Cunningham, Steven E. Cwirla, Warren C. Delano, Mark S. Dennis, William J. Dower, Michael D. Finucane, Marc Fransen, Christian M. Gates, Jason A. Hoffman, Mark Isalan, Pirjo Laakkonen, Henry B. Lowman, James D. Marks, David J. Matthews, Kentaro Miyazaki, Paolo Monaci, Ulrik Nielsen, Kimmo Porkka, Erkki Ruoslahti, Marjorie Russel, Peter J. Schatz, Sachdev Sidhu, Mihriban Tuna, Natalie G.M. Vlachakis, Christopher R.
Wagstrom, Gregory A Weiss, Derek N. Woolfson
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"...this is a nicely presented, readable text in which most people who use the technique in the laboratory will be able to find something useful. Richard J. Reece, University of Manchester."
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1: Russel, Lowman and Clackson: Introduction to phage biology and phage display
2: Sidhu and Weiss: Constructing phage display libraries by oligonucleotide-directed mutagenesis
3: Miyazaki and Arnold: In vitro DNA recombination
4: Dennis and Lowman: Phage selection strategies for improved affinity and specificity of proteins and peptides
5: DeLano and Cunningham: Rapid screening of phage displayed protein binding affinities by phage ELISA
6: Cwirla et al: Identification of novel ligands for receptors using recombinant peptide libraries
7: Matthews and Ballinger: Substrate phage display
8: Tuna et al: Protease-based selection of stably folded proteins and protein domains from phage display libraries
9: Isalan and Choo: Phage display of zinc fingers and other nucleic acid-binding motifs
10: Hoffman et al: In vivo and ex vivo selections using phage-displayed libraries
11: Monaci and Cortese: Screening phage libraries with sera
12: Jespers and Fransen: Interaction cloning using cDNA libraries displayed on phage
13: Bradbury and Marks: Phage antibody libraries
14: Neilson and Marks: Affinity maturation of phage antibodies
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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