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Flow Cytometry
A Practical Approach
Third Edition
Edited by Michael Ormerod
296 pages
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numerous figures
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246x189mm
978-0-19-963824-6
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Paperback
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18 May 2000
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This item is printed to order. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Updated volume with an additional apoptosis chapter
- Invaluable to both beginner and expert
- New design
- Over 50 protocols and 99 figures.
Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application
in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.Readership: Research and clinical laboratories.
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Edited by Michael Ormerod, Scientific Consultant Contributors:
David Barnett, Dept. of Haematology, Royal Hallamshire Hospital, Sheffield; Alison E. Bell, Dept. of Surgery, New Medical School, Newcastle-upon-Tyne, NE2 4HH; Nigel P. Carter, Sanger Institute, Hinxton Hall, Hinxton, Cambridge, CB10 1RQ; Derek C. Davies, Flow Cytometry Laboratory, ICRF, Lincoln Inn Fields, London Wc2A 3PX; Stephen F. Garner, National Blood Service East Anglia Centre and Division of Transfusion Medicine University of Cambridge, Long Road, Cambridge CB2 2PT; Jan W. Gratama, Dept. of Clinical and Tumour Immunology, Daniel de Hood Cancer Center, PO Box 5201, 3008 AE
Rotterdam, The Netherlands; Cheryl L. Green, HematoLogics Inc C/o Fred Hutchinson Cancer Research Center Seattle, WA USA; Terry Hoy University of Wales College of Medicine, Dept. of Haematology, Heath Park, Cardiff CF4 4XN; Carl H. June, Dept. of Molecular and Cellular Engineering, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA; Jorgen K. Larsen, Finsen Laboratory, Finsen Center, Rigshospitalet, Copenhagen University Hospital, Dept. 8621, Strandboulevarden 49, DK-2100 Copenhagen O, Denmark.; Michael R. Loken, HematoLogics Inc C/o Fred Hutchinson Cancer Research Center Seattle, WA USA; Mark W. Lowdell, BMT Unit, Dept. of Academic Haematology, The Royal Free Hospital Medical School, Pond Street, London NW3 1YD; Simon P. Monard, Trudeau Institute, Algonquin Avenue,
Saranac Lake, NY 12983, USA; Michael G. Ormerod, 34 Wray Park Road, Reigate, Surrey RH2 ODE; Martin Poot, University of Washington, Dept. of Pathology, Box 357705, Seattle WA 98195, USA; Peter S. Rabinovitch, University of Washington, Dept. of Pathology, Box 357705, Seattle WA 98195, USA; Brian K. Shenton, Dept. of Surgery, New Medical School, Newcastle upon Tyne, NE2 4HH; Denise A. Wells, HematoLogics, Inc., c/o Fred Hutchinson Cancer Research Center Seattle, WA USA; George D. Wilson, Gray Laboratory Cancer Research Trust, PO Box 100, Mount Vernon Hospital, Northwood HA6 2JR; Bryan D. Young, ICRF Medical Oncology Unit, St. Bartholomews Hospital, Charterhouse Square London, EC1A 7BE
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"This book will be useful to those people who wish to begin exploring the fascinating and attractive world of flow cytometry and also to those who need to update their knowledge of flow cytometry protocols in mammalian cell biology. It is also an excellent reference book on flow cytometry for use in the laboratory." - Cell Biology International
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Introduction to the principles of flow cytometry
Fluorescence and fluorescence technology
Preparing of suspensions of single cells
Flow sorting
Immunofluorescence of surface markers
Analysis of DNA - general methods
Further clinical applications
Quality control in the clinical laboratory
Measurement of cytoplasmic and nuclear antigens
Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method
Chromosome analysis and sorting by flow cytometry
Intracellular ionised calcium, magnesium, membrane potential and pH
Flow cytometry in the study of apoptosis
Further applications to cell and molecular biology
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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