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Kinetic Analysis of Macromolecules
A Practical Approach
Edited by Kenneth Johnson
276 pages
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numerous line figures, 13 halftones
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246x189mm
978-0-19-852493-9
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Hardback
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25 September 2003
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Clear descriptions of kinetics
- Concepts illustrated with examples
- Clear diagrams with practical information
- Coverage of proteins and nucleic acids
- Written by the experts in each field
Modern enzymology can no longer rely upon indirect inferences derived by steady state kinetic analysis because these are often misleading. Rather, in the era of 'New Enzymology', reactions at the active sites of enzyme are monitored by direct measurement of individual steps in the sequence to establish the pathway of reactions occurring. These quantitative methods can be directly related to high resolution structural information and define the underlying kinetic and thermodynamic basis for catalysis. The goal of a comprehensive kinetic analysis is to identify each step in the reaction pathway, define the rate and equilibrium constants for each step, identify all conformational and chemical intermediates, identify the rate-limiting steps, and ultimately
establish the basis for enzymatic specificity and efficiency. This volume contains chapters contributed by the leading experts in the application of modern kinetic methods to complex problems in the study of catalysis by both proteins and RNA catalysts. Each chapter focuses on a unique system and the novel methods used to obtain definitive data that can be directly applied to structural information, giving the much-needed practical details that are often omitted in the general literature. Readers will benefit greatly from the richness of each chapter in describing what is most important in solving the most difficult problems in modern enzymology. Readership: Academic researchers - Gain insights into obtaining
definitive data on enzymes
Graduate students in Biochem. & Mol. Biol - Learn methods to obtain definitive data on enzymes
Pharmaceutical Company researchers - Gain insights into obtaining definitive data on enzymes
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Edited by Kenneth Johnson, Roger Williams Centennial Professor of Biochemistry, The University of Texas at Austin and Founder and President of Kin Tek Corporation Contributors:
Kenneth A. Johnson, Karen S. Anderson, Gauri M.Dhavan, Mark R. Chance, Michael Brenowitz, Philip C. Bevukacqua, Trevor S. Brown, Durga M. Chadalavada, Amy D. Parente, Reiko Yajima, Smita S. Patel, Rajiv P. Bandwar, Mikhail K. Levin, Martin R. Webb, Dorothy Beckett, Bruce Allan Palfey, Yansheng Wei, Lusong Luo, Wenhai Zhang, Debra Dunaway
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"a useful book ... the information gained cannot be easily acquired otherwise. A useful contribution." - Doodys Publishers Club
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1: Johnson: Introduction to kinetic analysis of enzyme systems
2: Anderson: Detection and characterisation of enzyme intermediates
3: Dhavan, Change and Brenowitz: Kinetic analysis of DNA-Protein Interactions by time-resolved synchrotron X-ray footprinting
4: Bevilacqua et al: Kinetic analysis of ribozyme cleavage
5: Patel, Bandwar and Levin: Transient-state kinetics and computational analysis of transcription initiation
6: Webb: A fluorescent sensor to assay inorganic phosphate
7: Goody: What to do if there is no signal
8: Beckett: The biotin regulatory system-kinetic, themodynamic and structural interrogation of a biological switch
9: Palfey: Time resolved spectral analysis
10: Mariano et al: Kinetic analysis of enzyme mutants for identification of catalytic residues
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