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Readership: Postgraduates and researchers in biochemistry, molecular biology, oncology, pathology.
Edited by Ian McKay and Irene Leigh, both at the Department of Experimental Dermatology, London Hospital Medical College
"'for anyone planning to tackle these techniques ... this book is an invaluable source of hard-earned practical knowledge and experience from the front' Louis C. Mahadevan, King's College, Trends in Cell Biology, Vol. 3, December 1993"
"''this book is strongly recommended to anyone involved in growth-factor research' C. Jane Robinson, National Institute for Biological Standards and Control, Potters Bar, TIBTECH, March 1994 (Vol. 12)"
1: I.A. McKay: Types of growth factor activity: detection and characterization of new growth factor activities 2: E.J.J. Van Zoelen, B. Lelaey, N. Van der Burg, and D. Huylebroeck: Detection of polypeptide growth factors: application of specific bio-assays and PCR technology 3: A.N. Corps, and K.D. Brown: Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors 4: P. Sanjeeva Reddy and B.H. Cochran: Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization 5: J.M.W. Slack: Embryological assays for growth factors 6: R.T. Ballock and A.B. Roberts: The role of TGF- in bone growth and bone repair 7: R.J. Akhurst: Localization of growth factor mRNA in tissue sections by in-situ hybridization 8: P. Robinson, P. Whitehead, and W. Hume: Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP) 9: T.J. Dudgeon, J.M. Clements, and M.G. Hunter: Expression of growth factors in microbial systems 10: S.J. Capper: Immunoassays for growth factors 11: C. Linge and M.R. Green: Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis Appendices
