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Affinity Separations
A Practical Approach
Edited by Paul Matejtschuk
272 pages
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halftones, line figures, tables
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234x156mm
978-0-19-963550-4
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Paperback
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05 June 1997
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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Affinity Separations: A Practical Approach is a new collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents
detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. It is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work. Readership: Post-graduate and post-doctoral researchers and academic staff in analytical research and general bioscience laboratories.
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Edited by Paul Matejtschuk, Doctor, Research and Development, BioProducts Laboratory, Hertfordshire Contributors:
Chenna R. Chakka, Wichita State University, KS, USA; Richard D. Cummings, University of Oklahoma. OK, USA; M. A. J. Godfrey, Bio Products Laboratory, Elstree, UK; E. Lundgren, University of Umea, Sweden; A. Lydiatt, University of Birmingham, UK; Paul Matejtschuk, Bio Products Laboratory, Elstree, UK; S. B. Mohan, University of Birmingham, UK; John E. More, Bio Products Laboratory, Elstree, UK; Jacky Rott, Bio Products Laboratory, Elstree, UK; Satish Sharma, Upjohn Laboratories, MI, USA; Ram P. Singhal, Wichita State University, KS,
USA; Donald J. Winzor, University of Queensland, Australia
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1: Recent developments in affinity separation technologies
2: Quantitative affinity chromatography
3: Affinity separations of proteins
4: Affinity separation of nucleic acids
5.: Affinity chromatography of oligosaccharides and glycopeptides
6: Immunoaffinity and IgG receptor technologies
7: Designer affinity purifications of recombinant proteins
8: Affinity separations of cells
Appendix: list of suppliers
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