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Proteolytic Enzymes
A Practical Approach
Second Edition
Edited by Rob Beynon and J. S. Bond
358 pages
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15 halftones, numerous line figures
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246x189mm
978-0-19-963662-4
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Paperback
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11 January 2001
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Includes biological role of proteases and role of proteases as a research tool
Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The
topics covered are: nomenclature and classification; purification; assay methods; determination of mechanism; inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing; limited proteolysis as a structural probe; synthetic function. This book will be as invaluable as the first edition in providing ideas and protocols for scientists either studying proteases or using proteases as a research tool.Readership: Any scientist studying proteases or using proteases as tools in their research.
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Edited by Rob Beynon, Department of Veterinary Preclinical Studies, University of Liverpool, and J. S. Bond, Department of Biochemistry and Molecular Biology, The Pennsylvania State University Contributors:
Alan J. Barrett, MRC Peptidase Laboratory, Department of Immunology, The Babraham Institute, Cambridge; Sherwin Wilk, Department of Pharmacology, Mount Sinai School of Medicine, New York; Gautam Sarath, Michael Zeech & Alan R. Penheiter, Protein Core Facility, Centre for Biotechnology and Department of Biochemistry, University of Nebraska- Lincoln, USA; Benn M. Dunn, Department of Biochemistry & Molecular Biology,
University of Florida College of Medicine; Guy Salvesen, The Burnham Institute, USA; Hideaki Nagase, Kennedy Institute of Rheumatology, Imperian College School of Medicine, London; Bernhard Kuster, Andrej Shevchenko & Matthias Mann, Centre for Experimental BioInformatics , Odense University, Denmark; John D. Shannon, Biomolecular Research Facility, University of Virginia School of Medicine, USA; Michael J. North, Department of Biological Sciences, The University of Stirling, Scotland; Robert J. Beynon, Department of Veterinary Preclinical Sciences, University of Liverpool; Simon J. Hubbard, Department of Biomolecular Sciences, UMIST, Manchester; Volker Kasche, Technical University at Hamburg-Harburg, Germany
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""the second edition of Proteolytic enzymes;a practical approach is a welcome update to the volume of the same name ... good background to newcomers and students in the field, and will serve as a reference book for anyone working in ths area of research," Biochemistry and Molecular Biology"
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Proteolytic Enzymes: nomenclature and classification
Purification of Proteolytic Enzymes
Protease Assay Methods
Determination of Protease Mechanism
Inhibition of Proteolytic Enzymes
Finding, Purification and Characterization of Natural Protease Inhibitors
Mass Spectrometry of Proteolysis-derived Peptides for Protein Identification
Using Proteinases for Edman Sequence Analysis and Peptide Mapping
Prevention of Unwanted Proteolysis
Proteolysis of Native Proteins as a Structural Probe
Proteases in Peptide Synthesis
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