|
|
|
|
DNA-Protein Interactions
A Practical Approach
Edited by Andrew Travers and Malcolm Buckle
398 pages
|
4 pp colour plates, numerous black and white figures
|
246x189mm
978-0-19-963692-1
|
Hardback
|
03 August 2000
|
|
This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
|
|
|
- New design
- Covers a multi-disciplinary approach to biological problems
- Vital for the study of macromolecular DNA-protein complexes
- Over 70 protocols
- Well illustrated with some colour figures
DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are
covered.Readership: Postgraduates & research scientists working on gene transcription and chromatin structure.
|
|
|
Edited by Andrew Travers, MRC Laboratory of Molecular Biology, Cambridge, and Malcolm Buckle, Institut Pasteur, Paris Cedex, France Contributors:
Reinhard Grisshammer, Christian Kambach, Christopher G. Tate, MRC Laboratory of Molecular Biology,Cambridge; Marc Leng, Jean-Marc Malinge, Annie Schwartz, Centre de Biophysique Moleculaire, FRANCE; Dominique Payet, Centre d'Immunologie de Marseille Luminy, France; Len Lutter, Christopher E. abik and Herbert R. Halvorson, Molecular Biology Research Program, Henry Ford Hospital, USA; Louise Fairall, Memmo Buttinelli and Gianna Panetta, MRC Laboratory of Molecular Biology, Cambridge;
Stephen Diekmann, F. Stuhmeier, R. M. Clegg, A. Hillisch, Institute for Molecular Biotechnology, Germany; Jorg Langowski and M. TewesBiophysics of Macromolecules Division, Germany; C. Rivetti, Istituto di Scienze Biochimiche, Universita di Parma, Italy; Martin Guthold, Computer Science Department, Department of Physics and Astronomy, University of North Carolina, USA; Carlos Bustamante, Department of Chemistry, Institute of Molecular Biology, University of Oregon, USA; A. Cooper, Chemistry Department, Glasgow University; Konstantin Brodolin, Laboratory Molecular Genetics of Microorganisms Institute of Molecular Genetic Moscow; Peter Becker, Institut fur Physiologische Chemie, Universitat Munchen, Germany; Annie Kolb, Institut Pasteur 25, France; Tamara Belyaeva and Nigel Savery, School
of Biochemistry, The University of Birmingham, U.K.; M. Charlier and Mélanie spotheim-Maurizot, Centre de Biophysique Moleculaire, France; Malcom Buckle, Christopher Place and Iain K. Pemberton, Institut Pasteur, France; Johannes Geiselmann, Frédéric Boccard, Sylvie Déthiollaz, Manuel Engelhorn, Laboratoire du controlede l'expression génique, Université Joseph Fourier France; Judith Smith, MRC Laboratory of Molecular Biology, Cambridge; Andrew Travers, MRC Laboratory of Molecular Biology, Cambridge; Henri Buc, Bianca Sclavi, Pascal Roux, Institut Pasteur, France; Peter Stockley, Bjorn Persson, Department of Biology, University of Leeds; Michael D. Brenowitz and A.K.M.M. Mollah, Albert Einstein College of Medicine Department of BioChemistry, USA; David Millar and E.H.Z. Thompson, The
Scripps Research Institute, USA; Iain Pemberton, Institut Pasteur, France; Richard H. Ebright, Tae-Kyng Kim, Thierry Lagrange, Danny Reinberg, Howard Hughes Medical Institute, Rutgers University, USA; Nikolai Naryshkin, Howard Hughes Medical Institute, Waksman Institute and Department of Chemistry, Rutgers University, USA; Eric Le Cam, Etienne Delain, Laboratoire de Microscopie Moléculaire et Cullulaire, Institut Gustave-Roussy, FRANCE; Eric Larquet, Station Centrale de Microscopie Electronique, Instit Pasteur, France; Francoise Culard, Centre de Biophysique Moléculaire CNRS, France; Jean A. H. Cognet, Laboratoire de Physico-chimie Biomoléculaire et Cellulaire, Université Pierre et Marie Curie, France; William T. McAllister, Dmitry Temiakov, Pamela E. Karasavas, Department of Microbiology
and Immunology, Morse Institute for Molecular Genetics, State University of New York, USA
|
|
|
Expression Systems
Gel electrophoresis and bending in cisplatin-modified linear DNA
Use of DNA microcircles in protein-DNA binding studies
Use of topology to measure protein-induced DNA bend and unwinding angles
Bandshift, gel retardation, or electrophoretic mobility shift assays
Practical aspects of Fluorescence Resonance Energy Transfer (FRET) and its applications in nucleic acid biochemistry
Determination of DNA-ligand interactions by fluorescence correlation spectroscopy
DNA wrapping in E. Coli RNA polymerase open promotor complexes revealed by scanning force microscopy
Microcalorimetry of protein-DNA interactions
DNA-protein cross-linking by formaldehyde: a probe for RNAP-open promoter complex structure
Solid-phase DNAse I footprinting
Hydroxyl radical footprinting
Radiolytic cleavage of DNA: Mapping of the Protein Interaction Sites
UV - Laser photoreactivity of nucleoprotein complexes in vitro
In vivo UV-laser footprinting
Digitization and quantitative analysis of footprinting gels
Mapping histone positions in chromatin by protein-directed DNA cross-linking and cleavage
Kinetics of DNA-protein interactions
Kinetics of DNA interactions studied by surface plasmon resonance spectroscopy
Quantitative DNase I Kinetics Footprinting
Real time fluorescence anisotropy for studying protein-DNA interactions
Analysis of protein-DNA interactions in complex nucleoprotein assemblies
Site-specific protein-DNA photocrosslinking
Visualization of protein-DNA complexes by cryo em and on grids
Characterization of T7 RNA polymerase protein-DNA interactions during the initiation and elongation phases
|
|
|
|
|
|
|
|
|
Recently Viewed
|
|
|
Howard Skempton
Vocal score
£3.35
|
|
|
|
|
|
Volume 3
M. Proffitt, John Simpson
£40.00
|
|
|
|
|
Cecilia McDowall
Vocal score
£2.15
|
|
|
|
|
The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
|
|
