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Immunoassays
A Practical Approach
Edited by James Gosling
324 pages
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numerous line illustrations
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246x189mm
978-0-19-963710-2
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Paperback
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15 June 2000
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Up-to-date techniques written by uniquely qualified contributors
- Contains over 75 protocols
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common
interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.Readership: Researchers in: universities, hospitals, research institutes, governments and regulatory agencies, service and manufacturing industries.
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Edited by James Gosling, Department of Biochemistry, National University of Ireland Contributors:
Wadjdi G. Abdul Ahad, Beckman Coulter Inc., 200 S. Kraemer Blvd., Brea CA 92822, USA; John Banks, MAFF Central Science Laboratory, Sand Hutton, York YO4 1LZ; Martin G. Brett, HTI Bio-Services Inc., 10326 Roselle St. San Diego, CA 92121, USA; M. Qasim Chaudhry, MAFF Central Science Laboratory, Sand Hutton, York YO4 1LZ; John Coley, Immunology Section, Unilever Research, Colworth House, Sharnbrook Bedford MK44 1LQ; Eric Ezan, CEA Service de Pharmacologie et d'Immunologie, CE-Saclay 91191, Gif-sur-Yvette, France; Anthony Forde, National Diagnostic
Centre, National University of Ireland Galway, Galway, Ireland; James P. Gosling, Department of Biochemistry and National Diagnostic Centre, National University of Ireland Galway, Galway, Ireland; Jacques Grassi, CEA, Service de Pharmacologie et d'Immunologie, CE-Saclay 91191, Gif-sur-Yvette, France; Marian Kane, Immunodiagnostics Group, National Diagnostic Centre, National University of Ireland Galway, Galway, Ireland; A. Barry J. Nix, School of Mathematics, Cardiff University, Senghennydd Road, PO Box 926, Cardiff CF2 4YH; Robert S. Matson, Advanced Technology Centre, Beckman-Coulter Inc., 4300 N. Harbour Blvd., D-20-A Fullerton, CA 92834-3100, USA; Peter O'Fegan, National Diagnostic Centre, National University of Ireland Galway, Galway, Ireland; John Seth, Department of Clinical
Biochemistry, The Royal Infirmary of Edinburgh NHS Trust, Lauriston Place, Edinburgh, EH3 9YW; David G. Wild, Ortho-Clinical diagnostics, Forest Farm, Whitchurch, Cardiff CF4 7YT
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""This book is very useful not only for immunologists but also for amny specialists in chemistry and biology and is certainly recommendable" Immunological Investigations"
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Analysis by specific binding
Raising antibodies
Choosing and characterizing antibodies
Labels and endpoints
Solid-phase reagents
Standard and immunogens
Optimization
Validation
Data processing
Quality assurance
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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