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Zebrafish
Christiane Nusslein-Volhard and Ralf Dahm
322 pages
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numerous line drawings and halftones, 8pp colour plates
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246x189mm
978-0-19-963808-6
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Paperback
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19 September 2002
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- 73 detailed protocols, taking the reader step-by-step through experimental procedures
- Numerous figures and colour plates, for ease of intepretation
- Comprehensive appendix on all existing mutant strains: a unique resource
The zebrafish has become one of the most important model organisms to study biologial processes in vivo. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease.
The next few years will see the completion of large scale initiatives that exploit the zebrafish as a model system for the understanding of gene function in vertebrates, including the sequencing of the genome. The zebrafish will therefore play an increasingly important role in the future of biomedical research. Whole
genome sequencing projects, such as the human genome project, have led to the isolation of tens of thousands of genes for which the in vivo function is unknown. It is therefore likely that an increasing number of researchers will turn to organisms such as the zebrafish to understand the in vivo requirement for the proteins these genes encode.
Recent technical advances now allow the rapid testing of in vivo function of as yet uncharacterised genes in zebrafish in large numbers and at a speed that is impossible in other systems. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols
describing the most powerful techniques.Readership: All current zebrafish researchers; developmental geneticists, cell and developmental biologists; fish breeders
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Christiane Nusslein-Volhard, Department of Genetics, Max-Planck-Institut fur Entwicklungsbiologie, and Ralf Dahm, Department of Genetics, Max-Planck-Institut fur Entwicklungsbiologie Contributors:
Dr Michael Brand, Dr Ralf Dahm, Dr Hans Georg Fronhöfer, Dr Robert Geisler, Dr Darren Gilmour, Dr Michael Granato, Dr Jason Jessen, Dr Don Kane, Dr Yasuyuki Kishimoto, Dr Shuo Lin, Professor Christiane Nüsslein-Volhard, Dr Francisco Pelegri, Dr Thomas Schilling, Dr Stefan Schulte-Merker
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Nusslein-Volhard, Gilmour and Dahm: Introduction
1: Brand, Granato and Nusslein-Volhard: Keeping and raising zebrafish
2: Schulte-Merker: Looking at embryos
3: Schilling: The morphology of larval and adult zebrafish
4: Kane and Kiskimoto: Cell labelling and transplantation techniques
5: Gilmour, Jessen and Lin: Manipulating gene expression in the zebrafish
6: Pelegri: Mutagenesis
7: Geisler: Mapping and cloning
Appendices1: Dahm: Atlas of embryonic stages of development in the zebrafish
2: Frohnhofer: Table of zebrafish mutations
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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