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Spectrophotometry and Spectrofluorimetry
A Practical Approach
Second Edition
Edited by Mike Gore
386 pages
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numerous figures
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234x156mm
978-0-19-963812-3
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Paperback
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04 May 2000
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This item is printed to order. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Subjects covered relevant to laboratory work.
- Bridges the gap between undergraduate and postgraduate studies.
- Vital reference source.
- Very well illustrated with over 110 figures.
Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic
techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.Readership: Postgraduate researchers in biochemistry, chemistry and biomedical sciences working in areas of academia and
industry.
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Edited by Mike Gore, Institute of Biomolecular Sciences, University of Southampton Contributors:
Robert K. Poole, Krebs Inst. for Biomolecular Research, Dept. of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN; Uldis Kalnenieks, Inst. Of Microbiology and Biotechnology, University of Latvia, Kronvalda Boulevard 4, Riga, LV 1586, Latvia; Arthur G. Szabo, School of Physical Sciences, University of Windsor, 401 Sunset Avenue, Windsor, Canada N9B 3P4; Thomas D. Bradrick, Optical Spectrosocpy Section/LCB/NHLBI, Bldg. 10, Rm 5D14, MSC 1412, National Institutes of Health, Bethesda, MD 20892
- 1412, USA; J.E. Churchich, Dept. of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, PRC; Alison Roger, Dept. of Chemistry, University of Warwick, Coventry CB4 7AL; Matthew A. Ismail, Dept. of Chemistry, University of Warwick, Coventry CB4 7AL; Wlodek M. Bujalowski, Dept. of Human Biological Chemistry and Genetics and The Sealy Centre for Structural Biology, The University of Texas Medical Branch at Galveston, Room 5.138D Medical Research Building, 301 University Boulevard, Galveston, TX 77555-1053, USA; Maria J. Jezewska, Dept. of Human Biological Chemistry & Genetics and The Sealy Centre for Structural Biology, The University of Texas Medical Branch at Galveston, Room 5.138D Medical Research Building, 301 University Boulevard, Galveston, TX
77555-1053, USA; Athel Cornish-Bowden, Institut Fédératif "Biologie Structurale et Microbiologie", CNRS-LCB, 31 Chemin Joseph-Aiguier, B.P.71, 13402 Marseille Cedex 20, France; Tim Mantle, Dept. of Biochemistry, Trinity College, Dublin 2, Ireland; David A. Harris, Dept. of Biochemistry, South Parks Road, Oxford OX1 3QU; M. T. Wilson, Dept. of Biological and Chemical Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex C04 3SQ; Juave Torres, Dept. of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT; M.G. Gore, Institute of Biomolecular Sciences, Division of Biochemistry, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, S016 7PX; Steve Bottomley, Dept. of Biochemistry and Molecular Biology,
Monash University, Clayton, Vic. 3168, Australia; Michael G. Carey, Applied Photophysics Ltd., 203/205 Kingston Road, Leatherhead, Kent, KT22 7PB; C.L. Bashford, Dept. of Cell and Molecular Sciences, Division of Biochemistry, St. George's Hospital Medical School, Cranmer Terrace, London SW17 ORE; Maurice Eftink, Dept. of Chemistry, Uni. of Mississippi, MS, 38677, USA; Haripada Maity, Research Associate, Dept. of Chemistry, 112 Coulter Hall, Uni. of Mississippi, MS, 38677, USA; John SantaLucia, Jnr., Dept. of Chemistry, Wayne State University, Detroit, MI 48202, USA
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"'...Practical advice is given by experts in the different topics, together with lists of useful literature'Librairie"
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Introduction to light absorption; visible and ultraviolet spectra
Fluorescence principles and measurement
Time resolved fluorescence and phosphorescence spectroscopy
An introduction to circular dichroism
Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
Steady state kinetics
Spectrophotometric assays
Stopped flow spectroscopy
Stopped flow fluorescence
Stopped flow circular dichroism
Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
Use of optical spectroscopic methods to study the thermodynamic stability of proteins
The use of spectroscopic techniques in the study of protein stability
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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