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Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-protein interaction methods are also covered in detail. Inducible gene expression in plants as well as expression and analysis of recombinant proteins, and analysis of protein import into chloroplasts are covered as well as techniques for fractionation of plant tissue for biochemical analyses and the study of protein-protein interactions with the yeast two-hybrid system. A range of approaches for using antibodies as tools are also described including the use of antibody phage display libraries. The final section on emerging technologies describes methodologies for calcium imaging and for the spatial and temporal analysis of reporter genes such as luciferase and green fluorescent protein. The final area covers a range of experimental procedures for moss, which is emerging as a new model organism.
Readership: Graduate students and researchers in molecular biology and plant sciences.
Philip M Gilmartin, Director, Centre for Plant Sciences, University of Leeds, and Chris Bowler, Head, Laboratory of Molecular Plant Biology, Stazione Zoologica, Napoli, Italy
"The chapters are well written, with sufficient background detail and a step-by-step guide to each technique. Overall, this will be a useful manual for any plant molecular biology laboratory." - Microbiology Today
Part 1: Gene Expression 1: Transcript analysis 2: In situ hybridization 3: DNA microarrays for gene expression analysis 4: DNA-protein interactions 5: Inducible gene expression in plants Part 2: Gene Product Analysis 6: Expression of recombinant proteins 7: Import of proteins into isolated chloroplasts and thylakoid membranes 8: Fractionation of plant tissue for biochemical analysis 9: Studying protein-protein interactions with the yeast two-hybrid system 10: Antibody techniques 11: Antibody phage display libraries Part 3: Emerging Technologies 12: Spatial and temporal measurements of calcium ions in living plant cells 13: Reporter genes for plant cells 14: Moss gene technology
