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Enzyme Assays
Second Edition
Edited by Robert Eisenthal and Michael Danson
302 pages
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2 halftones, numerous figures
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246x189mm
978-0-19-963821-5
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Hardback
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11 April 2002
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Revised and updated version of a highly popular book
- Provides techniques that have wide applications throughout the biomolecular sciences
- Combines general principles with practical methods
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.
Building upon the highly popular first edition, this book combines revised or rewritten chapters with
entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.
This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life
sciences.Readership: Researchers in biochemistry and molecular biology, and all life sciences
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Edited by Robert Eisenthal, Department of Biology and Biochemistry, University of Bath, UK, and Michael Danson, Department of Biology and Biochemistry, University of Bath, UK Contributors:
Keith Tipton, Robert John, Kelvin Hughes, Shabih Syed, J. B. Clark, Keith Brocklehurst, Gunter Rothe, Nicholas Price, Mark Martin, David Hayes, Athel Cornish-Bowden, R. D. Hurst, Geoff Mellor, Lewis Stephens
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"Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes." - Natural Product Reports
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1: Keith F Tipton: Principles of enzyme assay and kinetic studies
2: Robert A John: Photometric assays
3: Kelvin T Hughes: Radiometric assays
4: Shabih E H Syed: High performance liquid chromatography assays
5: J B Clark: Electrochemical assays: the oxygen electrode
6: R D Hurst and J B Clark: Electrochemical assays: the nitric oxide electrode
7: Keith Brocklehurst: Electrochemical assays: the pH-stat
8: Gunter M Rothe: Enzyme assays after gel electrophoresis
9: Nicholas C Price and Lewis Stevens: Techniques for enzyme extraction
10: Mark T Martin: Determination of active site concentration
11: David Hayes and Geoff Mellor: High throughput screening - considerations for enzyme assays
12: Athel Cornish-Bowden: Statistical analysis of enzyme kinetic data
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