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Plant Cell Biology
A Practical Approach
Second Edition
Edited by Chris Hawes and Beatrice Satiat-Jeunemaitre
364 pages
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4 plates, 18 halftones, 14 line figures
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246x189mm
978-0-19-963865-9
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Paperback
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31 May 2001
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book
concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology. Readership: Advanced undergraduates, postgraduates and research staff in plant cell biology
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Edited by Chris Hawes, Research School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, and Beatrice Satiat-Jeunemaitre, Institut des Sciences Vegetales, Gif-sur-Yvette, France Contributors:
ELISON BLANCAFLOR, Biology Department, Penn State University, USAPETRA BOEVINK, Scottish Crop Research Institute, Invergowrie, Dundee, UKSPENCER BROWN, Institut des Sciences Végétales, CNRS, Gif-sur-Yvette, FranceTEODORO COBA DE LA PENA, Faculdad de Ciencias, Universidad de Vigo, SpainJURGEN DENECKE, School of Biology, University of Leeds, UKMARK FRICKER, Department of Plant Sciences, Oxford University, UKSIMON
GILROY, Biology Department, Penn State University, USAKARL OTTO GREULICH, Institut für Molekulare Biotechnologie, Jena, GermanyJANE HADLINGTON, School of Biology, University of Leeds, UKCHRIS HAWES, School of r slogical and Molecular Sciences, Oxford Brookes University, UKGISILBERT HINZ, Ruprecht-Karts-Universitat Heidelberg, Heidelberg, GermanyULRIKE HOMMAN, Pflanzenphysiologisches Institut der Universität Göttingen, GermanyMICHAEL KNOBLAUCH, Institut fur Allgemeine Botanik und Pflanzenphysiologie, Giessen, GermanyANDREW LEITCH, School of Biological Sciences, Queen Mary and Westfield
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"... an excellent guide for advanced students, postgraduates and researchers to handle techniques in plant cell biology." - Journal of Plant Physiology "The layout of the book is clear, with highly informative illustrations ... For anyone curious about cell biology, this book provides an excellent introduction to the major relevant techniques and works well as a laboratory manual." - Trends in Plant Science, 2002
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Introduction to optical microscopy for plant cell biology
Fluorescent probes for living plant cells
Flow cytometry
Transient expression, a tool to address questions in plant cell biology
The green fluorescent protein (GFP) as reporter in plant cells
Microinjection
Micromanipulation by laser microbeam and optical tweezers
Electrophysiological methods: monitoring exo- and endocytosis in real time
Plant histology
Immunocytochemistry for light microscopy
Electron microscopy
In Situ hybridization
Organelle isolation
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