A Laboratory Guide to the Mammalian Embryo

This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the Mouse Embryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.

Edited by David K. Gardner, Director of Research and Development, Michelle Lane, Senior Scientist, both at Colorado Center for Reproductive Medicine, and Andrew J. Watson, Associate Professor, Department of Physiology, University of Western Ontario

Preface
Acknowledgements
List of Contributors
Abbreviations
1: Norah Spears, Evelyn E. Telfer and Alison A. Murray: Follicle development in vitro
2: Michelle Lane and David Gardner: Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery
3: David Gardner and Michelle Lane: Culture of the mammalian preimplantation embryo
4: Denny Sakkas, Michelle Lane and David Gardner: Assessment of embryo development and viability
5: Gianpiero Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, Zev Rosenwaks: Micromanipulation: ICSI
6: Joyce Harper and Alpesh Doshi: Micromanipulation: Biopsy
7: Michelle Lane, Jay Baltz and David Gardner: Analysis of intracellular ions: pH and calcium
8: David Gardner and Michelle Lane: Nutrient uptake and metabolite production and enzyme activity and regulation
9: Don Rieger: Metabolic Pathway
10: Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten: Confocal imaging of structural molecules in mammalian gametes
11: Leeanda Wilton: Fluorescent in situ hybridization
12: W. A. King, D. H. Betts, W. Slimane and P.K. Basur: Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos
13: C. Wrenzycki, A.J. Watson, and M. Calder: Microscale RNA isolation from mammalian embryos
14: P. A. De Sousa and A. J. Watson: Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
15: Dave Natale: Characterization of novel genes during early development by application of differential display RT-PCR
16: K. E. Latham, G. Nourjanova, K. Wigglesworth and J. J. Eppig: Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells
17: Lisa C Barcroft, D Holstead Jones-Taggart and Andrew J Watson: Antisense disruption of gene expression in the preimplantation embryo
18: M. A. Sirard and C. Robert: Gene subtraction and analysis
19: Daniel Brison Anthony D Metcalfe, Debra J Bloor, Helen R Hunter, Gerard Brady, Susan J Kimber: Analysis of apoptosis in the preimplantation embryo
20: D. P. Baluch, C. M. Pauken, and D. G. Capco: Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo
21: Susan E. Lazendorf, Catherine A. Boyd and Diane L. Wright: Human Embryonic Stem
22: Makoto Nagano: Spermatogonial Transplantion
23: Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut: Somatic Cell Nuclear Transfer
24: Tetsunori Mukaida and Magosaburo Kasai: Cryobiology; slow freezing vitrification of embryos