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In Situ Hybridization in Neurobiology
Advances in Methodology
James H. Eberwine, Karen L. Valentino, and Jack D. Barchas
212 pages
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colour and halftone illustrations, line figures, tables
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234x156mm
978-0-19-507507-6
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Hardback
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03 March 1994
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This item will be ordered from OUP USA. Items ordered from OUP USA are despatched and charged as soon as we receive them, which is normally within 2 weeks
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- Essential to researchers in neurosciences
- Focuses on combined uses of in situ hybridization with immunohistochemistry and receptor autoradiography
In situ hybridization has become an important and widely used research tool for neurobiologists. This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs and both proteins and mRNAs within the same section, and methods for using in situ hybridization technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized
probes, and the combined use of in situ hybridization and immunocytochemistry is also covered. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.Readership: Neurobiologists.
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James H. Eberwine, Departments of Pharmacology and Psychiatry, University of Pennsylvania School of Medicine, Karen L. Valentino, Research Scientist, Neurex Corporation, and Jack D. Barchas, Associate Dean for Research and Development, University of California, Los Angeles School of Medicine
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1: James Eberwine: Methodological considerations in the utilization of In situ hybridization
2: C. Dominique Toran-Allerand and Rajesh C. Miranda: Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography
3: P.C. Emson, B. Heppelman, and S.M. Augood: Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons
4: Frank Baldino, Jr., Elaine Robbins, and Michael E. Lewis: Enzyme histochemical detection of neuronal mRNA
5: Sandra L. Petersen and Sue McCrone: Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry
6: Phyllis M. Wise, Kathryn Scarbrough, Sufen Chiu, Gregg H. Larson, Nancy G. Weiland, Jonathan M. Lloyd, David A. Hinkle, and Aihua Cai: Assessment of peptide secretion and gene expression within individual cells
7: Olcay Yeralan, ShyXing Zhang, Cynthis Rovnaghi, and W. Sue Y. Griffin: Quantitative micro-In situ hybridation
8: Marie-Francoise Chesselet and Linda T. Weiss-Wunder: Quantification of In situ hybridization histochemistry
9: James L. Roberts and Nancy Levin: Analysis of transcription levels using intervening sequence In situ hybridization
10: Rudolph E. Tanzi and Bradley T. Hyman: Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes
11: Omar Bagasra, Thikkavarapu Sesshamma, and Roger J. Pomerantz: In situ polymerase chain reaction: A powerful new methodology
12: Kevin Miyashiro, Richard Finnell, Ann Ouyang, Scott Mackler, Corinne Spencer, and James Eberwine: In situ transcription: Methodology and multi-faceted applications
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