HIV: A Practical Approach

• Detailed protocols providing a step-by-step guide to modern HIV research.
• Internationally recognized contributors
• Comprehensive review of virological and immunological methods
• First volume to review biochemistry, molecular biology, and drug discovery methods.

Readership: These books provide instructions in virological, biochemical, molecular biological, and immunological methods of use for HIV researchers in academic institutions, medical schools, and the pharmaceutical industry.

Edited by Jonathan Karn, MRC Laboratory of Molecular Biology, Cambridge

"HIV: a Practical Approach is the latest two-volume offering from the Practical Approach series and provides the first comprehensive methodology for the HIV field. As such, it can be recommended strongly to all active bench scientists in the field as well as to desk-bound research directors who wish to keep in touch ... well worth the effort." - James C. Neil, University of Glasgow Veterinary School, Molecular Medicine Today, October 1996

"The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interaction between cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratory working with HIV." - M.A. Koch, Zentralblatt fur Bakteriologie 1996

Section 1: Growth and isolation of HIV
1.: Biological safety when working with HIV
2.: Virological and molecular genetic techniques for studies of established HIV isolates
3.: Isolation and biological characterization of primary HIV isolates
4.: HIV infection of macrophages
5.: HIV infection of dendritic cells
6.: Vectors based on HIV
Section 2: Analysis of HIV infection
7.: Quantitative assays for virus neutralization
8.: Detection of HIV entry into cells
9.: Detection of viral DNA by PCR
10.: Detection of viral RNA by PCR
11.: Sequence analysis of virus variability based on PCR
Section 3: Immunology
12.: Humoral responses and detection during HIV infection
13.: Quantification of CD4-positive T lymphocytes
14.: CD-4 positive T-cell responses in HIV-1 infection
15.: Lymphokine responses to HIV infection
16.: HIV-1 specific cytotoxic T-lymphocyte responses and ADCC