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Macrophages
Practical Approach Series
Edited by Donna M. Paulnock
228 pages
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numerous halftones and line figures
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246x189mm
978-0-19-963688-4
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Paperback
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14 September 2000
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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Macrophages are an important part of the immune response and are characterized by their ability to phagocytose foreign matter. However the difficulties involved in macrophage isolation mean they are some of the body's least explored cells. Macrophage Methodology describes how to isolate moderate to high yields of viable cells from a variety of specific tissue sites under both normal and pathological conditions and then goes on to give protocols for macrophage purification. The third chapter covers techniques used to identify and measure endocytic and phagocytic capabilities using immunochemistry and fluorescent analysis. Chapter four identifies the key issues relating to the study of macrophages as antigen presenting cells and has protocols for the
major assays used to measure antigen processing and presentation. Also covered are the theoretical and practical issues related to the processing and presentation of intracellular pathogens for which macrophages are the major host cell. The methods described for measuring macrophage secretory products concentrate on bioassays for molecules where no ELISA is available. The next two chapters cover measuring macrophage activity in vitro and in vivo. Finally methods are described for the analysis of gene expression in macrophages. A variety of broad techniques have been brought together in one affordable volume to make Macrophage Methodology an essential buy for anyone studying macrophages. Readership: Immunologists
and cell biologists studying macrophages.
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Edited by Donna M. Paulnock, Department of Medical Microbiology and Immunology, University of Wisconsin Medical School Contributors:
Diana Lopez, Mary Ellen Handel-Fernandez, Department of Microbiology and Immunology, PO Box 016960 (R138), Miami, USA; Sandra Gessani, Laura Fantuzzi, Patrizia Puddu, Fillippo Belardelli, Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy; Leane Peiser, Peter J Gough, Elizabeth Darley, Siamon Gordon, Sir William Dunn School of Pathology, University of Oxford; Paul M. Kaye, Department of Infectious and Tropical Disease, London School of Hygiene and Tropical Medicine, University of London; Alberto
Mantovani, Giancarlo Bianchi, Walter Luini, Andrea Doni, Pietro Transidico, Silvano Sozzani, Istituo di Richerche Farmacologiche Mario Negri, Milan, Italy; Luigi Varesio Maria Carla Bosco, Tiziana Musso, Luca Carta, Laboratorio di Biologia Molecolare, Istituto Gianinni, Genova, Italy; Nico van Rooijen, Ester van Kesteren, Department of Cell Biology and Immunology, Faculty of Medicine, Free University, The Netherlands; Donna M. Paulnock, Joyce E.S. Doan, Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, USA; Thomas A Hamilton, Department of Immunology, Cleveland Clinic Foundation, USA
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Isolation of macrophages from tissues, fluids, and immune response sites
Purification of macrophages
Characterization of macrophage antigens and receptors by immunochemistry and fluorescent analaysis
Analysis of antigen processing and presentation
Macrophage secretory products
Analysis of macrophage lytic function
Analysis of macrophage activity in vivo
Analysis of gene expression in mononuclear phagocytes
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