Cell Separation

Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.

Readership: Graduate and post-doctoral students, academics and industrial researchers in the general area of cell biology.

Edited by Derek Fisher, Molecular Cell Pathology, Royal Free Hospital School of Medicine, Gillian E. Francis, Molecular Cell Pathology, Royal Free Hospital School of Medicine, and David Rickwood, Department of Biology, University of Essex

"'...the book is a perfect introduction manual for all laboratory workers in the life sciences.'" - Aslib Book Guide, Vol.64, no.3, March 1999.

"'...the book gives detailed and reliable descriptions of most available methods for preparation and separation of isolated cells from tissues. It should certainly find its place in laboratories that use or plan to use methods for cell separation' Cell Biology International Vol.24, No.6"

1: Preparation of single cell suspensions
2: Fractionation of cells by sedimentation methods
3: Centrifugal elutriation
Appendix: Simulation of centrifugal elutriation for the Beckman E-6B chamber
4: Separation and frustrationation of cells by partitioning in aqueous two-phase systems
Appendix: Manually operated counter current distribution apparatus
5: Separation cells by flow cytometry
6: Immuno methods: magnetic, columns and panning techniques
7: Separation of cells using free flow electrophoresis
Appendix: Suppliers