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FISH (Fluorescence In Situ Hybridization)
Barbara G Beatty, Sabine Mai, and Jeremy A Squire
274 pages
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numerous figures, halftones and tables; 16pp colour plates
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246x189mm
978-0-19-963884-0
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Paperback
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04 July 2002
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This item is printed to order and supplied on a firm sale basis. Items which are printed to order are normally despatched and charged within 5-10 days.
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- Comprehensive coverage by international experts, providing topical information on many aspects of FISH
- Many full colour illustrations, assisting the reader in interpretation
- One text containing all the information needed for FISH, from basic to sophisticated applications
- Trouble shooting protocols included, particularly useful for this sensitive technique
- 109 easy to follow protocols, as suitable for start-up laboratories as for experienced FISH users
This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene
mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular
diagnostics and genetics.Readership: Scientists, technologists and students in the fields of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.
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Barbara G Beatty, Health Sciences Research Facility, University of Vermont, USA, Sabine Mai, Manitoba Institute of Cell Biology, Winnipeg, Canada, and Jeremy A Squire, Ontario Cancer Institute, Toronto, Canada Contributors:
Jane Bayani, Barbara Beatty, Michael Bittner, Patricia Bray-Ward, T Cremer, M Cremer, Arthur A Glatfelter, Gerald C Gooden, F Habermann, H Heng, Yuan Jiang, Carol V Johnson, Stefan Joos, J Khan, E Kolomietz, J B Lawrence, Peter Lichter, Sabine Mai, P Marrano, John A McNeil, Paul Meltzer, Lao H Saal, Steve Scherer, L Schermelleh, Carsten Schwänen, Lindsay S Shopland, I Solovei, Jeremy
Squire, Rose Tam, J Walter, Francis Wiener.
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"As a resource for both the researcher and the clinician, this book should serve admirably. It should be a welcome addition to any technical library." - Proteomics "... offers an excellent array of protocols aimed at the laboratory scientist." - Proteomics "... the book does an excellent job of describing purification and enzymatic labeling techniques of probe DNA, and offers a comprehensive list of sources for various FISH probes and reagents." - Proteomics "... useful as a laboratory manual." - Proteomics
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1: Beatty, Mai, Squire: Introduction
2: Bray-Ward: Labelling Methods
3: Beatty, Scherer: FISH Mapping
4: Mai, Wiener: Murine FISH
5: Heng: Fiber FISH
6: Tam et al: RNA FISH
7: Cremer et al: Three Dimensional FISH
8: Lichter, Joos, Schwanen: CGH
9: Squire, Marrano, Kolomietz: Clinical Cytogenetics
10: Bayani, Squire: Multicolour FISH and SKY
11: Khan et al: cDNA Microarrays and FISH
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The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.
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