Antibody Engineering

• State-of-the-art protocols for generating antibodies of high affinity and specificity
• Chapters contributed by leading researchers in the field

Readership: Postgraduates, post-doctoral researchers, and other academic staff involved in both clinical and molecular biological applications of antibodies.

Edited by John McCafferty, Hennie Hoogenboom, and Dave Chiswell, all at Cambridge Antibody Technology Limited

"This book is very useful for practising antibody engineers, providing extra information that enables them to adopt new techniques or expand into new areas. ... this is a useful and interesting resource for practising antibody engineers and I will make sure my students have access to it." - SGM Quarterly, August 1997

1: Construction and use of antibody gene repertoires
2: Affinity maturation of antibodies using phage display
3: Human antibody repertoires in transgenic mice: manupulation and transfer of YACs
4: Measuring antibody affinities in solution
5: Measuring antibody affinities using Bio-sensors
6: Analysis of human antibody sequences
7: Rodent to human antibodies by CDR grafting
8: Rodent to human antibodies by phage display
9: Choosing and manipulating effector functions
10: Secretion and purification of antibody fragments expressed in bacteria
11: Production of single chain Fv monomers and multimers
12: Expression of immunoglobulin genes in mammalian cells
13: In vitro linkage of antibody fragments into dimeric and multimeric forms